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Publikationstyp
Wissenschaftlicher Artikel
Erscheinungsjahr
2020
Detection and characterization of hepatitis E virus genotype 3 in wastewater and urban surface waters in Germany
Detection and characterization of hepatitis E virus genotype 3 in wastewater and urban surface waters in Germany
Autor:innen
Herausgeber
Quelle
Food and Environmental Virology
12 (2020)
12 (2020)
Schlagwörter
Monitoring, Abwasser, Oberflächengewässer
Zitation
BEYER, Sophia, Regina GNIRRS, Hans-Christoph SELINKA und Regine SZEWZYK, 2020. Detection and characterization of hepatitis E virus genotype 3 in wastewater and urban surface waters in Germany. Food and Environmental Virology [online]. 2020. Bd. 12 (2020). DOI 10.60810/openumwelt-209. Verfügbar unter: https://openumwelt.de/handle/123456789/4240
Zusammenfassung englisch
In highly populated areas, environmental surveillance of wastewater and surface waters is a key factor to control the circulation of viruses and risks for public health. Hepatitis E virus (HEV) genotype 3 is considered as an emerging pathogen in industrialized countries. Therefore, this study was carried out to determine the prevalence of HEV in environmental waters in urban and suburban regions in Germany. HEV was monitored in water samples using quantitative RT-PCR (RT-qPCR) and nested RT-PCR without or with virus concentration via polyethylene glycol precipitation or ultracentrifugation. By RT-qPCR, 84-100% of influent samples of wastewater treatment plants were positive for HEV RNA. Genotypes HEV-3c and 3f were identified in wastewater, with HEV-3c being the most prevalent genotype. These data correlate with subtypes identified earlier in patients from the same area. Comparison of wastewater influent and effluent samples revealed a reduction of HEV RNA of about 1 log10 during passage through wastewater treatment plants. In addition, combined sewer overflows (CSOs) after heavy rainfalls were shown to release HEV RNA into surface waters. About 75% of urban river samples taken during these CSO events were positive for HEV RNA by RT-qPCR. In contrast, under normal weather conditions, only around 30% of river samples and 15% of samples from a bathing water located at an urban river were positive for HEV. Median concentrations of HEV RNA of all tested samples at this bathing water were below the limit of detection. Source: https://link.springer.com