Publikation:
Detection and characterization of hepatitis E virus genotype 3 in wastewater and urban surface waters in Germany

Kurzanzeige
dc.contributor.authorBeyer, Sophia
dc.contributor.authorGnirrs, Regina
dc.contributor.authorSelinka, Hans-Christoph
dc.contributor.authorSzewzyk, Regine
dc.date.accessioned2024-06-16T14:06:35Z
dc.date.available2024-06-16T14:06:35Z
dc.date.issued2020
dc.description.abstractIn highly populated areas, environmental surveillance of wastewater and surface waters is a key factor to control the circulation of viruses and risks for public health. Hepatitis E virus (HEV) genotype 3 is considered as an emerging pathogen in industrialized countries. Therefore, this study was carried out to determine the prevalence of HEV in environmental waters in urban and suburban regions in Germany. HEV was monitored in water samples using quantitative RT-PCR (RT-qPCR) and nested RT-PCR without or with virus concentration via polyethylene glycol precipitation or ultracentrifugation. By RT-qPCR, 84-100% of influent samples of wastewater treatment plants were positive for HEV RNA. Genotypes HEV-3c and 3f were identified in wastewater, with HEV-3c being the most prevalent genotype. These data correlate with subtypes identified earlier in patients from the same area. Comparison of wastewater influent and effluent samples revealed a reduction of HEV RNA of about 1 log10 during passage through wastewater treatment plants. In addition, combined sewer overflows (CSOs) after heavy rainfalls were shown to release HEV RNA into surface waters. About 75% of urban river samples taken during these CSO events were positive for HEV RNA by RT-qPCR. In contrast, under normal weather conditions, only around 30% of river samples and 15% of samples from a bathing water located at an urban river were positive for HEV. Median concentrations of HEV RNA of all tested samples at this bathing water were below the limit of detection. Source: https://link.springer.comen
dc.format.extent1 Onlineresource (pages 137-147)
dc.format.mediumonline resource
dc.identifier.doihttps://doi.org/10.60810/openumwelt-209
dc.identifier.urihttps://openumwelt.de/handle/123456789/4240
dc.language.isoeng
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectMonitoring
dc.subjectAbwasser
dc.subjectOberflächengewässer
dc.titleDetection and characterization of hepatitis E virus genotype 3 in wastewater and urban surface waters in Germany
dc.title.alternativeDetection and characterization of hepatitis E virus genotype 3 in wastewater and urban surface waters in Germany
dc.typeWissenschaftlicher Artikel
dc.type.dcmitext
dc.type.mediumcomputer
dspace.entity.typePublication
local.bibliographicCitation.journalTitleFood and Environmental Virology
local.bibliographicCitation.originalDOI10.1007/s12560-020-09424-2
local.bibliographicCitation.volume12 (2020)
local.collectionAufsätze
local.contributor.authorId02184802
local.identifier.catalogId02476544
local.ingest.leader05587naa a2200000uu 4500
local.jointTitleDETECTION AND CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN WASTEWATER AND URBAN SURFACE WATERS IN GERMANY
local.reviewtrue
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relation.isAuthorOfPublication.latestForDiscovery77e044cb-dfad-4a9d-a311-42e3fc22d951
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