Development of an extended OECD One-Generation Reproduction Test with Zebrafish (ZEOGRT) for the Identification of Endocrine Disruptors

Abstract

The aim of the project was to validate an extended one-generation reproduction test with zebrafish (ZEOGRT).The method is used to investigate potentially hormone-active substances and comprises an adult parental reproduction phase (F0), a complete filial generation (F1) and the hatching of a second filial generation (F2). The method can detect potential estrogenic and androgenic mechanisms of action and can be extended to other mechanisms of action (e.g. thyroid hormones). The project demonstrates both the applicability of the protocol and its successful transfer to international laboratories.