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Publication type
Wissenschaftlicher Artikel
Date
2020
'http://rightsstatements.org/vocab/InC/1.0/'

A tiered high-throughput screening approach for evaluation of estrogen and androgen receptor modulation by environmentally relevant bisphenol A substitutes

Authors
Keminer, Oliver
Teigeler, Matthias
Kohler, Manfred
Editor
Containing Item
The Science of the Total Environment
717 (2020)
Keywords
Bisphenol A
Citation
KEMINER, Oliver, Jürgen ARNING, Matthias TEIGELER und Manfred KOHLER, 2020. A tiered high-throughput screening approach for evaluation of estrogen and androgen receptor modulation by environmentally relevant bisphenol A substitutes. The Science of the Total Environment [online]. 2020. Bd. 717 (2020). DOI 10.60810/openumwelt-172. Verfügbar unter: https://openumwelt.de/handle/123456789/3913
Abstract english
Bisphenol A (BPA) is a high production volume chemical with a broad application spectrum. As an endocrine disrupting chemical, mainly by modulation of nuclear receptors (NRs), BPA has an adverse impact on organisms and is identified as a substance of very high concern under the European REACH regulation. Various BPA substitution candidates have been developed in recent years, however, information concerning the endocrine disrupting potential of these substances is still incomplete or missing. In this study, we intended to investigate the endocrine potential of BPA substitution candidates used in environmentally relevant applications such as thermal paper or epoxy resins. Based on an extensive literature and patent search, 33 environmentally relevant BPA substitution candidates were identified. In order to evaluate the endocrine potential of the BPA replacements, a screening cascade consisting of biochemical and cell-based assays was employed to investigate substance binding to the NRs estrogen receptor ÎÌ and Î2, as well as androgen receptor, co-activator recruitment and NR-mediated reporter gene activation. In addition, a computational docking approach for retrospective prediction of receptor binding was carried out. Our results show that some BPA substitution candidates, for which so far no or only very few data were available, possess a substantial endocrine disrupting potential (TDP, BPZ), while several substances (BPS, D-8, DD70, DMP-OH, TBSA, D4, CBDO, ISO, VITC, DPA, and DOPO) did not reveal any NR binding. © 2019 The Author(s).