Publikation:
Genome-wide DNA methylation sequencing identifies epigenetic perturbations in the upper airways under long-term exposure to moderate levels of ambient air pollution

Kurzanzeige
dc.contributor.authorMessingschlager, Marey
dc.contributor.authorBartel-Steinbach, Martina
dc.contributor.authorMackowiak, Sebastian D.
dc.contributor.authorStraff, Wolfgang
dc.contributor.otherSüring, Katrin
dc.date.accessioned2024-06-16T12:40:33Z
dc.date.available2024-06-16T12:40:33Z
dc.date.issued2023
dc.description.abstractWhile the link between exposure to high levels of ambient particulate matter (PM) and increased incidences of respiratory and cardiovascular diseases is widely recognized, recent epidemiological studies have shown that low PM concentrations are equally associated with adverse health effects. As DNA methylation is one of the main mechanisms by which cells regulate and stabilize gene expression, changes in the methylome could constitute early indicators of dysregulated signaling pathways. So far, little is known about PM-associated DNA methylation changes in the upper airways, the first point of contact between airborne pollutants and the human body. Here, we focused on cells of the upper respiratory tract and assessed their genome-wide DNA methylation pattern to explore exposure-associated early regulatory changes. Using a mobile epidemiological laboratory, nasal lavage samples were collected from a cohort of 60 adults that lived in districts with records of low (Simmerath) or moderate (Stuttgart) PM10 levels in Germany. PM10 concentrations were verified by particle measurements on the days of the sample collection and genome-wide DNA methylation was determined by enzymatic methyl sequencing at single-base resolution. We identified 231 differentially methylated regions (DMRs) between moderately and lowly PM10 exposed individuals. A high proportion of DMRs overlapped with regulatory elements, and DMR target genes were involved in pathways regulating cellular redox homeostasis and immune response. In addition, we found distinct changes in DNA methylation of the HOXA gene cluster whose methylation levels have previously been linked to air pollution exposure but also to carcinogenesis in several instances. The findings of this study suggest that regulatory changes in upper airway cells occur at PM10 levels below current European thresholds, some of which may be involved in the development of air pollution-related diseases. Quelle: Elsevieren
dc.format.extent1 Online-Ressource (14 pages)
dc.format.mediumonline resource
dc.identifier.doihttps://doi.org/10.60810/openumwelt-34
dc.identifier.urihttps://openumwelt.de/handle/123456789/1594
dc.language.isoeng
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectPM10
dc.titleGenome-wide DNA methylation sequencing identifies epigenetic perturbations in the upper airways under long-term exposure to moderate levels of ambient air pollution
dc.typeWissenschaftlicher Artikel
dc.type.dcmitext
dc.type.mediumcomputer
dspace.entity.typePublication
local.bibliographicCitation.journalTitleEnvironmental Research
local.bibliographicCitation.originalDOI10.1016/j.envres.2023.116413
local.bibliographicCitation.volume233 (2023)
local.collectionAufsätze
local.contributor.authorId02192923
local.contributor.authorId(DE-588)1299854494
local.contributor.authorId02150013
local.contributor.authorId02192925
local.contributor.otherId02141249
local.identifier.catalogId02501100
local.ingest.leader06073naa a2200000uu 4500
local.jointTitleGENOMEWIDE DNA METHYLATION SEQUENCING IDENTIFIES EPIGENETIC PERTURBATIONS IN THE UPPER AIRWAYS UNDER LONGTERM EXPOSURE TO MODERATE LEVELS OF AMBIENT AIR POLLUTION
local.reviewtrue
local.sourcecatalog
local.source.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.de
local.staffPublicationtrue
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relation.isAuthorOfPublication.latestForDiscoveryebb2db05-15bd-462c-b089-6c1da912257f
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