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Veröffentlichung Cold atmospheric pressure plasma comb - a physical approach for pediculosis treatment(2018) Bosch, Lars ten; Habedank, Birgit; Siebert, DominikPediculosis, that is the infestation of humans with Pediculus humanus capitis (head lice), poses a worldwide problem that is as old as mankind itself. Over the centuries, man has developed a variety of remedies, all of which have ultimately culminated in the use of chemical agents. Some of these remedies are known to produce successful results. A large portion of the effective remedies used to kill lice and their eggs contain insecticides, but there is an increasing number of reports of head lice populations revealing an increased resistance. This study presents an alternative treatment approach, the efficacy of which is based on physical effects. Cold atmospheric pressure plasmas have successfully shown their formidably wide application range within the field of plasma medicine. This study presents a plasma device in its current stage of development that is engineered as a consumer product to enable an alternative physical and insecticide-free option for the treatment of pediculosis. An efficacy study concerning different developmental stages of P. humanus humanus is presented. P. humanus humanus was chosen as a substitute test organism for P. humanus capitis due to possible laboratory rearing and high anatomic similarity. The study shows how a single stroke of the plasma device over a hair strand (approximately 22 cm in length with a weight of 1.5 g) led to mortality rates of 68.3% (50.0; 79.7) (95% CI) in the juvenile test group, a mortality rate of approx. 67.7% (54.9; 78.8) (95% CI) in the female test group, and approx. 46.7% (28.3; 65.7) (95% CI) in the male test group. When single eggs were introduced directly into the plasma for approx. 1 s, younger eggs (0-2 d) showed a higher mortality of 66.7% (42.7; 82.7) than the older (4-6 d) eggs, with 16.7% (5.6; 34.7) (CI). Furthermore, the results of a risk assessment of the device are described. The article concludes with necessary handling instructions as well as further developmental steps, derived from the results of the efficacy and the risk assessment study. Quelle: https://www.mdpi.comVeröffentlichung Erratum: ten Bosch et al. Cold atmospheric pressure plasma comb - a physical approach for pediculosis treatment(2020) Bosch, Lars ten; Habedank, Birgit; Siebert, DominikVeröffentlichung Light sheet fluorescence microscopy for the investigation of blood-sucking arthropods dyed via artificial membrane feeding(2022) Bosch, Lars ten; Candeo, Alessia; Bassi, Andrea; Valentini, Gianluca; Habedank, Birgit; Gerhard, ChristophPhysical methods to control pest arthropods are increasing in importance, but detailed knowledge of the effects of some of these methods on the target organisms is lacking. The aim of this study was to use light sheet fluorescence microscopy (LSFM) in anatomical studies of blood-sucking arthropods in vivo to assess the suitability of this method to investigate the morphological structures of arthropods and changes in these structures over time, using the human louse Pediculus humanus (Phthiraptera: Pediculidae) as sample organism. Plasma treatment was used as an example of a procedure employed to control arthropods. The lice were prepared using an artificial membrane feeding method involving the ingestion of human blood alone and human blood with an added fluorescent dye in vitro. It was shown that such staining leads to a notable enhancement of the imaging contrast with respect to unstained whole lice and internal organs that can normally not be viewed by transmission microscopy but which become visible by this approach. Some lice were subjected to plasma treatment to inflict damage to the organisms, which were then compared to untreated lice. Using LSFM, a change in morphology due to plasma treatment was observed. These results demonstrate that fluorescence staining coupled with LSFM represents a powerful and straightforward method enabling the investigation of the morphology - including anatomy - of blood-sucking lice and other arthropods. © The Author(s) 2022.