Publikation:
Light sheet fluorescence microscopy for the investigation of blood-sucking arthropods dyed via artificial membrane feeding

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dc.contributor.authorBosch, Lars ten
dc.contributor.authorCandeo, Alessia
dc.contributor.authorBassi, Andrea
dc.contributor.authorValentini, Gianluca
dc.contributor.authorHabedank, Birgit
dc.contributor.authorGerhard, Christoph
dc.date.accessioned2024-06-16T13:17:53Z
dc.date.available2024-06-16T13:17:53Z
dc.date.issued2022
dc.description.abstractPhysical methods to control pest arthropods are increasing in importance, but detailed knowledge of the effects of some of these methods on the target organisms is lacking. The aim of this study was to use light sheet fluorescence microscopy (LSFM) in anatomical studies of blood-sucking arthropods in vivo to assess the suitability of this method to investigate the morphological structures of arthropods and changes in these structures over time, using the human louse Pediculus humanus (Phthiraptera: Pediculidae) as sample organism. Plasma treatment was used as an example of a procedure employed to control arthropods. The lice were prepared using an artificial membrane feeding method involving the ingestion of human blood alone and human blood with an added fluorescent dye in vitro. It was shown that such staining leads to a notable enhancement of the imaging contrast with respect to unstained whole lice and internal organs that can normally not be viewed by transmission microscopy but which become visible by this approach. Some lice were subjected to plasma treatment to inflict damage to the organisms, which were then compared to untreated lice. Using LSFM, a change in morphology due to plasma treatment was observed. These results demonstrate that fluorescence staining coupled with LSFM represents a powerful and straightforward method enabling the investigation of the morphology - including anatomy - of blood-sucking lice and other arthropods. © The Author(s) 2022.en
dc.format.extent1 Onlineresource (8 pages)
dc.format.mediumonline resource
dc.identifier.doihttps://doi.org/10.60810/openumwelt-1959
dc.identifier.urihttps://openumwelt.de/handle/123456789/2798
dc.language.isoeng
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBlut
dc.subjectIn-Vivo
dc.titleLight sheet fluorescence microscopy for the investigation of blood-sucking arthropods dyed via artificial membrane feeding
dc.typeWissenschaftlicher Artikel
dc.type.dcmitext
dc.type.mediumcomputer
dspace.entity.typePublication
local.bibliographicCitation.journalTitleParasites & Vectors
local.bibliographicCitation.originalDOI10.1186/s13071-022-05157-2
local.bibliographicCitation.volume15 (2022)
local.collectionAufsätze
local.contributor.authorId02178906
local.contributor.authorId02189742
local.contributor.authorId02191222
local.contributor.authorId02191223
local.contributor.authorId02191224
local.identifier.catalogId02490344
local.ingest.leader05868naa a2200000uu 4500
local.jointTitleLIGHT SHEET FLUORESCENCE MICROSCOPY FOR THE INVESTIGATION OF BLOODSUCKING ARTHROPODS DYED VIA ARTIFICIAL MEMBRANE FEEDING
local.reviewtrue
local.sourcecatalog
local.source.urihttp://creativecommons.org/licenses/by/4.0/deed.de
local.staffPublicationtrue
relation.isAuthorOfPublicatione0dce9b2-6821-4de9-a071-33e1a5ec63cd
relation.isAuthorOfPublication.latestForDiscoverye0dce9b2-6821-4de9-a071-33e1a5ec63cd
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