Publikation:
Impact of in vitro digested zinc oxide nanoparticles on intestinal model systems

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dc.contributor.authorMittag, Anna
dc.contributor.authorHöra, Christian
dc.contributor.authorSinger, Alina
dc.contributor.authorKämpfe, Alexander David
dc.date.accessioned2024-06-16T12:50:34Z
dc.date.available2024-06-16T12:50:34Z
dc.date.issued2022
dc.description.abstractBackground: Zinc oxide nanoparticles (ZnO NP) offer beneficial properties for many applications, especially in the food sector. Consequently, as part of the human food chain, they are taken up orally. The toxicological evaluation of orally ingested ZnO NP is still controversial. In addition, their physicochemical properties can change during digestion, which leads to an altered biological behaviour. Therefore, the aim of our study was to investigate the fate of two different sized ZnO NP (< 50 nm and < 100 nm) during in vitro digestion and their effects on model systems of the intestinal barrier. Differentiated Caco-2 cells were used in mono- and coculture with mucus-producing HT29-MTX cells. The cellular uptake, the impact on the monolayer barrier integrity and cytotoxic effects were investigated after 24 h exposure to 123-614 mikroM ZnO NP. Results: In vitro digested ZnO NP went through a morphological and chemical transformation with about 70% free zinc ions after the intestinal phase. The cellular zinc content increased dose-dependently up to threefold in the monoculture and fourfold in the coculture after treatment with digested ZnO NP. This led to reactive oxygen species but showed no impact on cellular organelles, the metabolic activity, and the mitochondrial membrane potential. Only very small amounts of zinc (< 0.7%) reached the basolateral area, which is due to the unmodified transepithelial electrical resistance, permeability, and cytoskeletal morphology. Conclusions: Our results reveal that digested and, therefore, modified ZnO NP interact with cells of an intact intestinal barrier. But this is not associated with serious cell damage. Quelle: Artikelen
dc.format.extent1 Online-Ressource (15 Seiten)
dc.format.mediumonline resource
dc.identifier.doihttps://doi.org/10.60810/openumwelt-1853
dc.identifier.urihttps://openumwelt.de/handle/123456789/1948
dc.language.isoeng
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectToxizität
dc.subjectZinkoxid
dc.titleImpact of in vitro digested zinc oxide nanoparticles on intestinal model systems
dc.typeWissenschaftlicher Artikel
dc.type.dcmitext
dc.type.mediumcomputer
dspace.entity.typePublication
local.bibliographicCitation.journalTitleParticle and fibre toxicology
local.bibliographicCitation.originalDOI10.1186/s12989-022-00479-6
local.bibliographicCitation.volume19 (2022), Heft 39
local.collectionAufsätze
local.contributor.authorId02188920
local.contributor.authorId(DE-588)1232455679
local.contributor.authorId02192134
local.identifier.catalogId02498371
local.ingest.leader05638naa a2200000uu 4500
local.jointTitleIMPACT OF IN VITRO DIGESTED ZINC OXIDE NANOPARTICLES ON INTESTINAL MODEL SYSTEMS
local.reviewtrue
local.sourcecatalog
local.source.urihttp://creativecommons.org/licenses/by/4.0/
local.staffPublicationtrue
relation.isAuthorOfPublication0c011c64-0492-4463-b27f-a4d7db2cf0e1
relation.isAuthorOfPublicationcddf45bc-0404-4c73-b2cb-b06e1599ff23
relation.isAuthorOfPublication.latestForDiscovery0c011c64-0492-4463-b27f-a4d7db2cf0e1
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