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Publikationstyp
Wissenschaftlicher Artikel
Erscheinungsjahr
2016
A collaborative evaluation of LC-MS/MS based methods for BMAA analysis: soluble bound BMAA found to be an important fraction
A collaborative evaluation of LC-MS/MS based methods for BMAA analysis: soluble bound BMAA found to be an important fraction
Autor:innen
Faassen, Elisabeth J.
Antoniou, Maria G.
Beekman-Lukassen, Wendy
Blahova, Lucie
Chernova, Ekaterina
Christophoridis, Christophoros
Combes, Audrey
Edwards, Christine
Harmsen, Joop
Herausgeber
Quelle
Marine Drugs
14 (2016), Heft 3
14 (2016), Heft 3
Schlagwörter
Phytoplankton, Meeresfrüchte
Zitation
FAASSEN, Elisabeth J., Maria G. ANTONIOU, Wendy BEEKMAN-LUKASSEN, Lucie BLAHOVA, Ekaterina CHERNOVA, Christophoros CHRISTOPHORIDIS, Audrey COMBES, Christine EDWARDS, Jutta FASTNER, Joop HARMSEN, Anastasia HISKIA, Leopold L. ILAG, Triantafyllos KALOUDIS, Srdjan LOPICIC, Miquel LÜRLING, Hanna MAZUR-MARZEC, Jussi MERILUOTO, Cristina POROJAN, Yehudit VINER-MOZZINI und Nadezda ZGUNA, 2016. A collaborative evaluation of LC-MS/MS based methods for BMAA analysis: soluble bound BMAA found to be an important fraction. Marine Drugs [online]. 2016. Bd. 14 (2016), Heft 3. DOI 10.60810/openumwelt-1228. Verfügbar unter: https://openumwelt.de/handle/123456789/7333
Zusammenfassung englisch
Exposure to â-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimers disease and Parkinsons disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D3BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D3BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.Quelle: http://www.mdpi.com