Publikation:
Nitrogen Limitation Promotes Accumulation and Suppresses Release of Cylindrospermopsins in Cells of Aphanizomenon Sp.

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dc.contributor.authorPreußel, Karina
dc.contributor.authorChorus, Ingrid
dc.contributor.authorFastner, Jutta
dc.date.accessioned2024-06-16T16:17:31Z
dc.date.available2024-06-16T16:17:31Z
dc.date.issued2014
dc.description.abstractAs the biosynthesis of cylindrospermopsin (CYN) is assumed to depend on nitrogen availability, this study investigated the impact of nitrogen availability on intra- and extracellular CYN and deoxy-CYN (D-CYN) contents in three Aphanizomenon strains from temperate waters. Nitrogen deficient (-N) cultures showed a prolonged growth phase and intracellular toxin accumulation by a factor of 2-6. In contrast, cultures with additional nitrate supply (+N) did not accumulate CYN within the cells. Instead, the maximum conceivable CYN release estimated for dead cells (identified by SYTOX® Green staining) was much lower than the concentrations of dissolved CYN actually observed, suggesting these cultures actively release CYN from intact cells. Furthermore, we found remarkably altered proportions of CYN to D-CYN: as batch cultures grew, the proportion of D-CYN increased by up to 40% in +N medium, whereas D-CYN remained constant or decreased slightly in -N medium. Since +N cultures showed similar toxin patterns as -P cultures with increased extracellular CYNs and higher proportion of D-CYN we conclude that nitrogen limitation may affect the way the cells economize resources, especially the yield from phosphorus pools, and that this has an impact on CYN production and release. For water management, these result imply that nutrient availability not only determines the abundance of potentially CYN-producing cyanobacteria, but also the amount of extracellular CYNs (challenging drinking-water treatment) as well as the ratio of D-CYN to CYN (affecting toxicity).<BR>Quelle: http://www.mdpi.com/en
dc.identifier.doihttps://doi.org/10.60810/openumwelt-578
dc.identifier.urihttps://openumwelt.de/handle/123456789/8335
dc.language.isoeng
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBlaualgen
dc.subjectFreiwerdung [Organismen]
dc.titleNitrogen Limitation Promotes Accumulation and Suppresses Release of Cylindrospermopsins in Cells of Aphanizomenon Sp.
dc.typeWissenschaftlicher Artikel
dspace.entity.typePublication
local.bibliographicCitation.journalTitleToxins
local.bibliographicCitation.originalDOI10.3390/toxins6102932
local.bibliographicCitation.volume6 (2014), H. 10, Seite 2932-2947
local.collectionAufsätze
local.contributor.authorId02131226
local.identifier.catalogId02417089
local.ingest.leader04908naa a2200000uu 4500
local.jointTitleNITROGEN LIMITATION PROMOTES ACCUMULATION AND SUPPRESSES RELEASE OF CYLINDROSPERMOPSINS IN CELLS OF APHANIZOMENON SP
local.sourcecatalog
local.source.urihttp://www.mdpi.com/2072-6651/6/10/2932
local.staffPublicationtrue
relation.isAuthorOfPublication8332d730-8fa3-45be-9068-9d8d61f864f6
relation.isAuthorOfPublication890ce9dc-f5f1-4671-a2de-a1aa7e420d64
relation.isAuthorOfPublication.latestForDiscovery8332d730-8fa3-45be-9068-9d8d61f864f6
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