Moos, Rebecca K.Kolossa-Gehring, MarikeApel, PetraAngerer, JürgenBrüning, ThomasKoch, Holger M.2024-06-162024-06-162013https://openumwelt.de/handle/123456789/8624Because of the ubiquitous presence and the extensive use of parabensand other phenolic substances (like triclosan, triclocarban or benzophenones) in personal care and consumer products, food and beverage processing, pharmaceuticals and disinfectants humans are constantly exposed to these substances. Some of these substances are suspected to affect the endocrine system of humans. The German Environmental Specimen Bank (ESBHum) - supervised and scientifically coordinated by the German Federal Environment Agency (UBA) - archives human specimens since 1985 and thus allows assessing trends of human exposure. We quantified the body burden to these substances (9 parabens and 6 other phenolic substances) by analyzing 660 urine samples of the ESBHum (24h-urines) from the years 1995, 1997, 1999, 2001, 2003, 2005-2009 and 2012; 60 samples per year, from 30 male and 30 female volunteers (age: 21-29 years). We applied a newly developed highly sensitive on-line HPLC-MS/MS method, for the determination of these substances in urine. Analytical limits of quantification were in the range from 0.5 to 2 Ţg/L. The total concentration of the analytes (free and conjugated species) was measured. All samples were blinded before analysis. In nearly all samples parabens could be quantified.Median concentrations over the different years were around 40 Ţg/L for methyl-, 2 Ţg/L for ethyl- and 5 Ţg/L for n-propyl paraben. For the parabens this mainly reflects their consumption volume being highest for methyl paraben, followed by ethyl-, propyl- and butyl paraben. Triclosan was detected in around 60% of all samples analyzed. Median benzophenone-3 levels fell from around 2.5 Ţg/L (1995-2003) to < LOQ (2005-2012). For all other analytes medians are below the LOQ. Over the years we observed rather constant levels of exposures, except for the decrease in benzophenone-3. This retrospective study provides first data on internal exposures to the above substances in Germany from 1995 till 2012. Quelle: http://ehp.niehs.nih.gov40 Vortragsfoliengraph. Darst.enghttp://rightsstatements.org/vocab/InC/1.0/Conference proceedings