Auflistung nach Autor:in "Koch, Holger Martin"

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A glyphosate exposure assessment among families in the Irish population - The IMAGE project
(2019) Connolly, Alison; Koch, Holger Martin; Conrad, André; Kolossa-Gehring, Marike
A glyphosate exposure assessment among families in the Irish population - The IMAGE project
(2019) Connolly, Alison; Koch, Holger Martin; Conrad, André; Kolossa-Gehring, Marike
A human biomonitoring study assessing glyphosate and Aminomethylphosphonic Acid (AMPA) exposures among farm and non-farm families
(2022) Connolly, Alison; Koch, Holger Martin; Bury, Daniel; Conrad, André; Kolossa-Gehring, Marike; Murawski, Aline
Glyphosate-based pesticides are the highest-volume used herbicides worldwide. International concerns regarding the potential human adverse effects of glyphosate exposures have heightened since IARC classified glyphosate as probably carcinogenic to humans. Human biomonitoring (HBM) studies have identified ubiquitous exposure to glyphosate and its main breakdown product, aminomethylphosphonic acid (AMPA), from environmental exposures. The IMAGE research project aimed to investigate farm and non-farm families' exposure to glyphosate while aligning with the Human Biomonitoring for Europe (HBM4EU) initiative. The study recruited non-farm and farm families (who use glyphosate on their farms). Each family member provided a urine sample that was analysed using gas chromatography coupled with tandem mass spectrometry, with a limit of quantification of 0.05 (micro)g/L for glyphosate and AMPA. In addition to general information on background exposures in farm and non-farm families, we investigated relationships in exposure between families and family members. We recruited 68 families, including 54 non-farm and 14 farm families (180 vs. 45 individuals). Some pesticide users (n = 14, all male farmers) had slightly elevated AMPA levels compared to other adult participants but, overall, we observed no significant differences between farm and non-farm families. The main metabolite, AMPA, was quantifiable in twice as many samples as glyphosate (61% vs. 32%), with a maximum concentration of 7.24 (micro)g/L vs. 3.21 (micro)g/L. Compared to previous studies, exposure levels were relatively low and far below current health-based guidance values (3% or less for glyphosate and AMPA). Study results suggest potential exposures from residential co-exposures or living with a pesticide user. This is the first study internationally to investigate glyphosate and AMPA across family members (farm and non-farm). We found comparably low glyphosate and AMPA exposures among these families. These results enhance our understanding of glyphosate exposures for different demographic groups and contribute to the scientific knowledge on exposures required for regulatory risk assessments and the re-evaluation of glyphosate in 2022 by the European Commission. © 2022 by the authors
A pilot study on the feasibility of European harmonized Human Biomonitoring: Strategies towards a common approach, challenges and opportunities
(2015) Casteleyn, Ludwine; Dumez, Birgit; Becker, Kerstin; Den Hond, Elly; Schoeters, Greet; Castaño, Argelia; Koch, Holger Martin; Angerer, Jürgen; Esteban, Marta; Exley, Karen; Sepai, Ovnair; Bloemen, Louis; Fiddicke, Ulrike; Horvath, Milena; Knudsen, Lisbeth E.; Joas, Anke; Joas, Reinhard; Biot, Pierre; Koppen, C.; Dewolf, M.-C.; Katsonouri, Andromachi; Hadjipanayis, Adamos; Cerna, Milena; Krskova, A.; Kolossa-Gehring, Marike; Nielsen, Jeanette K.S.; Jensen, J.F.; Rudnai, Peter; Közepesy, S.; Mulcahy, M.F.R.; Mannion, R.; Gutleb, Arno C.; Fischer, M.E.; Ligocka, Danuta; Jakubowski, M.; Reis, M.Fátima; Namorado, S.; Lupsa, Ioana-Rodica; Schwedler, Gerda; Gurzau, Anca Elena
In 2004theEuropeanCommissionandMemberStatesinitiatedactivitiestowardsaharmonizedap-
proach forHumanBiomonitoringsurveysthroughoutEurope.Themainobjectivewastosustainen-
vironmental healthpolicybybuildingacoherentandsustainableframeworkandbyincreasingthe
comparability ofdataacrosscountries.Apilotstudy totestcommonguidelinesforsettingupsurveys
wasconsideredakeystepinthisprocess.Throughabottom-upapproachthatincludedallstakeholders,
a jointstudyprotocolwaselaborated.
FromSeptember2011tillFebruary2012,17Europeancountriescollecteddatafrom1844mother-
child pairsintheframeofDEMOnstrationofastudytoCoordinateandPerformHumanBiomonitoring
on aEuropeanScale(DEMOCOPHES). Mercury inhairandurinarycadmiumandcotininewereselected
as biomarkersofexposurecoveredbysufficient analyticalexperience.PhthalatemetabolitesandBi-
sphenol Ainurinewereaddedtotakeintoaccountincreasingpublicandpoliticalawarenessfor
emerging typesofcontaminantsandtotestlessadvancedmarkers/markerscoveredbylessanalytical
experience.Extensiveeffortstowardschemo-analyticalcomparabilitywereincluded.
The pilotstudyshowed thatcommonapproachescanbefoundinacontextofconsiderablediffer-
ences withrespecttoexperienceandexpertize,socio-culturalbackground,economicsituationandna-
tional priorities.ItalsoevidencedthatcomparableHumanBiomonitoringresultscanbeobtainedinsuch
context.AEuropeannetworkwasbuilt,exchanging information,expertise andexperiences,andpro-
viding trainingonallaspectsofasurvey.Akeychallengewas finding therightbalancebetweenarigid
structure allowingmaximalcomparabilityanda flexibleapproachincreasingfeasibilityandcapacity
building. NextstepsinEuropeanharmonizationinHumanBiomonitoringsurveysincludetheestab-
lishment ofajointprocessforprioritizationofsubstancestocoverandbiomarkerstodevelop,linking
biomonitoring surveyswithhealthexaminationsurveysandwithresearch,andcopingwiththediverse
implementations ofEUregulationsandinternationalguidelineswithrespecttoethicsandprivacy.
©2014ElsevierInc.Allrightsreserved.
Analysis of new chemicals in the 5th German Environmental Survey 2014-2017
(2015) Kolossa-Gehring, Marike; Schwedler, Gerda; Apel, Gerda; Koch, Holger Martin; Seiwert, Margarete; Conrad, André; Schulz, Christine; Fiddicke, Ulrike
The German Environmental Surveys (GerES) are large scale population studies repeatedly been carried out since the mid-1980s. GerES evaluates pollutant body burdens in population-representative samples, the contribution of different media (air, drinking water, food) to the overall exposure, and links human biomonitoring (HBM) to health data. GerES is conducted in cooperation with the National Health Interview and Examination Surveys (NHIES) performed by the Robert Koch Institute. GerES V focuses on emerging substances with potential health relevance and/or assumed exposure of the general population (e.g. plasticizer alternatives like Hexamoll® DINCH and di-2-propylheptyl phthalate (DPHP), the solvents N-methyl- and N-ethyl-2-pyrrolidone (NMP/NEP), 6 parabens, Triclosan and the vulcanization accelerator 2-mercaptobenzothiazole (2-MBT)) which are measured in children and adolescents aged 3 to 17 years. A pilot study (n=39) was conducted in 2013 to validate instruments and field work procedures. GerES V field work started in 2014. The pilot study revealed large differences in exposure between chemicals and individuals. The percent of samples with markers above LOQ were: 100% for DEHP and DINCH, 97% for NEP and NMP, 55% for DPHP, 30% for Triclosan, 28% for 2-MBT and between 100% and 3% for different parabens. Maximum levels for methylparaben and N-propylparaben were 6.8 and 3.4 mg/l urine, respectively, applying a method with a LOQ of 0.5 ìg/l. All values were below the established or proposed Human Biomonitoring values of the German HBM Commission for DEHP, DINCH, DPHP, NEP, NMP and 2-MBT. However, only the main study supplies population representative data which can answer the question if subgroups might be exposed to critical levels as judged by a toxicological assessment of single or combined effects. GerES is funded by the Federal Ministry for the Environment, Nature Conservation, Building and Nuclear Safety (BMUB) and the Federal Ministry of Education and Research.In: 25th Annual Meeting: Exposures in an Evolving Environment: October 18 - 22, 2015 - Henderson, Nevada: final Abstract Book. International Societyof Exposure Science. Henderson,S.124
Analyzing terephthalate metabolites in human urine as biomarkers of exposure: Importance of selection of metabolites and deconjugation enzyme
(2018) Koch, Holger Martin; Lessmann, Frederik; Kolossa-Gehring, Marike; Swan, Shanna H.
Biomonitoring of the industrial alkyl pyrrolidone solvents NMP and NEP: specific metabolites in 24-hour urine samples from the German Environmental Specimen Bank (1996/2012)
(2014) Koch, Holger Martin; Ulrich, N.; Schröter-Kermani, Christa; Bader, M.; Käfferlein, H.U.; Brüning, T.; Kolossa-Gehring, Marike
N-Methyl-2-pyrrolidone (NMP) and N-ethyl-2-pyrrolidone (NEP) are multi-purpose organic solvents in
industry. Both are developmental and teratogenic toxicants in rodents. NMP is classified as a REACh substanceof very high concern. Because of their toxicological profile and their broad application resulting in a possibleexposure of the general population, NMP and NEP were chosen as target substances for the cooperationproject between the German Federal Ministry for the Environment, Nature Conservation, Building and NuclearSafety (BMUB) and the German Chemical Industry Association (VCI) aiming to establish human biomonitoring(HBM) methods for "newŁ substances of interest. NMP and NEP are metabolized to 5-hydroxy-N-alkyl-2-pyrrolidones (5-HNMP, 5-HNEP) and 2-hydroxy-N-alkylsuccinimides (2-HMSI, 2-HESI). We analyzed thesespecific metabolites in 24-hour urine samples from the German Environmental Specimen Bank. For thispurpose, 20 randomly selected samples collected in 1996 and in 2012, respectively, were analyzed by asensitive and specific GC-MS/MS method with isotope dilution quantification. We detected NMP metabolites in100% and NEP metabolites in 95% of all samples. Despite the considerable differences in the elimination halftimesof the alkyl pyrrolidone metabolites, the correlations between the metabolites were rather strong (NMP:r=0.51; NEP: r=0.67). An exposure determined through one metabolite is thus confirmed by the other129metabolite. Median NMP metabolite levels were comparable between 1996 (5-HNMP 50 ìg/L, 2-HMSI 46 ìg/L)and 2012 (5-HNMP 39 ìg/L, 2-HMSI 41 ìg/L). Surprisingly, urinary levels of NEP metabolites were approx. 10times higher in 1996 (5-HNEP 14 ìg/L, 2-HESI 42 ìg/L) as compared to 2012 (5-HNEP ~1 ìg/L, 2-HESI 5ìg/L). We would have expected a reverse trend for NEP since NEP has only recently been introduced into themarket as a substitute for NMP. The sources of past and present exposures to NMP and NEP warrant furtherinvestigations.
Quelle: 24th Annual Meeting ofThe International Society of Exposure Science: Exposure Science Integration to Protect Ecological Systems,Human Well-Being, and Occupational Health; Abstrct Book ISES 2014 / International Society of Exposure Science, Cincinnati: 2014, S. 128
BPA and risk assessment
(2020) Calafat, Antonia M.; Koch, Holger Martin; Andra, Syam S.; Kolossa-Gehring, Marike
Development of a multi-compartment pharmacokinetic model to characterize the exposure to Hexamoll® DINCH®
(2015) Schütze, Andre; Apel, Petra; Lorber, Matthew; Gawrych, Katarzyna; Kolossa-Gehring, Marike; Brüning, Thomas; Koch, Holger Martin
We developed and calibrated a multi compartment pharmacokinetic (PK) model to predict urinary concentrations after oral exposure of four specific DINCH metabolites: MINCH, OH-MINCH, cx-MINCH, and oxo-MINCH. This descriptive model has 4 compartments: a "stomachŁ (SC) compartment, a "holdingŁ (HC) compartment, a "bloodŁ (BC) compartment and a "bladderŁ (BLC) compartment. DINCH is assumed to first deposit into the SC, with transfer split between the HC and the BC. Unmetabolized DINCH from the HC then transfers to the BC. The DINCH metabolism is assumed to occur in the BC before excretion via the BLC. At each urination event, all the metabolite mass in the BLC is excreted. The model was calibrated using published urine metabolite data from 3 different male volunteers, each orally dosed with 50 mg DINCH. Full urine voids were taken for 48 h after dosage. The predicted values showed a good agreement with the observed urinary DINCH metabolite concentrations, with a Spearman correlation coefficient exceeding 0.7 for all oxidized metabolites. We showed the importance of a holding reservoir. Without it, a good agreement could not be found. We applied the model to a set of 24-h general population samples measured for DINCH metabolites. The model was unable to duplicate the ratio of metabolites seen in the 24-h samples. Two possibilities were offered to explain the difference: the exposure pattern in the general population did not match the oral exposure in the dosing experiments, or the long-term toxicokinetics of DINCH was not captured in the 48-h controlled dosing experiments.Quelle: http://www.sciencedirect.com
DINCH Exposure in Germany has become omnipresent and is further increasing - urinary data from the German Environmental Specimen Bank (1999-2017)
(2018) Koch, Holger Martin; Kasper-Sonnenberg, Monika; Weber, Till; Apel, Petra; Kolossa-Gehring, Marike
DPHP metabolites in urine samples of the German Environmental Specimen Bank from 1999 to 2012
(2014) Schütze, Andre; Gries, Wolfgang; Kolossa-Gehring, Marike; Apel, Petra; Schröter-Kermani, Christa; Brühning, Thomas; Leng, Gabriele; Koch, Holger Martin
The aim of this study was to investigate a possible time trend and status quo of
dipropylheptylphthalate (DPHP) exposure. DPHP is used as a substitute of other high molecular weightphthalates in high temperature applications (e.g cable wires, roofing membranes, etc.). DPHP was selected inthe cooperation project between the German Federal Ministry for Environment (BMU) and the German ChemicalIndustry Association (VCI) due to its listing as High Production Volume (HPV) chemical in the European Union.The BMU-VCI project establishes new human biomonitoring methods and biomarkers for fifty emergingsubstances. 300 urine samples (24-hour voids) from the German Environmental Specimen Bank were analyzedfor three specific, secondary oxidized DPHP metabolites (with hydroxyl, oxo and carboxy modifications of thealkyl side chain). Urine samples were collected in the years 1999, 2003, 2006, 2009 and 2012, 60 samples peryear, from 30 male and 30 female volunteers (age: 21-29 years). The samples were analyzed by liquid/liquidextraction followed by GC-HRMS, which enabled us to distinguish between DPHP and Di-iso-decyl phthalate(DiDP) metabolites. The limit of quantification was between 0.15 ìg/l and 0.3 ìg/l, depending on the metabolite.All samples were blinded before analysis. DPHP metabolites were not detected in the years 1999-2006.Thereafter, detection rates increased from 3.3% in 2009 to 21.7% in 2012. As expected, mono-oxo-propylheptylphthalate (oxo-MPHP) was the most abundant metabolite, with concentrations between Quelle: 24th Annual Meeting ofThe International Society of Exposure Science: Exposure Science Integration to Protect Ecological Systems,Human Well-Being, and Occupational Health; Abstrct Book ISES 2014 / International Society of Exposure Science, Cincinnati: 2014, S.128
From zero to one-hundred in 15 years
(2018) Koch, Holger Martin; Kasper-Sonnenberg, Monika; Weber, Till; Apel, Petra; Kolossa-Gehring, Marike
German Environmental Specimen Bank:evidence of increasing population exposures to the UV filters octocrylene and 2-ethylhexyl salicylate from 1996-2020
(2022) Bury, Daniel; Weber, Till; Koch, Holger Martin; Kolossa-Gehring, Marike
German human biomonitoring health-based guidance values and approaches to interpret internal exposure levels
(2023) Koch, Holger Martin; Apel, Petra; Röhl, Claudia; Kolossa-Gehring, Marike
Harmonization of human biomonitoring in Europe: the European Human Biomonitoring Initiative HBM4EU
(2018) Kolossa-Gehring, Marike; Fiddicke, Ulrike; Koch, Holger Martin; Esteban, Marta; Castaño, Argelia
Metabolites of the alkyl pyrrolidone solvents NMP and NEP in 24-h urine samples of the German Environmental Specimen Bank from 1991 to 2014
(2018) Ulrich, Nadin; Bury, Daniel; Koch, Holger Martin; Kolossa-Gehring, Marike; Rüther, Maria; Weber, Till
Purpose The aim of this study was to get a first overview of the exposure to the solvents and reproductive toxicants N-methyl-2-pyrrolidone (NMP) and N-ethyl-2-pyrrolidone (NEP) in Germany. NMP and NEP metabolite concentrations were determined in 540 24-h urine samples of the German Environmental Specimen Bank collected from 1991 to 2014. With these data we were able to investigate NMP/NEP exposures over time and to evaluate associated risks. Methods NMP metabolites 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) and NEP metabolites 5-hydroxy-N-ethyl-2-pyrrolidone (5-HNEP) and 2-hydroxy-N-ethylsuccinimide (2-HESI) were determined by stable isotope dilution analysis using solid phase extraction followed by derivatization (silylation) and GCâ€ÌEIâ€ÌMS/MS. Results We were able to quantify 5-HNMP and 2-HMSI in 98.0 and 99.6% and 5-HNEP and 2-HESI in 34.8 and 75.7% of the samples. Metabolite concentrations were rather steady over the timeframe investigated, even for NEP which has been introduced as an NMP substitute only in the last decade. Calculated median daily intakes in 2014 were 2.7 Ìg/kg bw/day for NMP and 1.1 Ìg/kg bw/day for NEP. For the combined risk assessment of NMP and NEP exposure, the hazard index based on the human biomonitoring assessment I values (HBM I values) was less than 0.1. Conclusions Based on the investigated subpopulation of the German population, individual and combined NMP and NEP exposures were within acceptable ranges in the investigated timeframe. Sources of NEP exposure in the 90s and 00s remain elusive. © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Metabolites of the substitute plasticiser Di-(2-ethylhexyl) terephthalate (DEHTP) in urine of children and adolescents investigated in the German Environmental Survey GerES V, 2014-2017
(2020) Conrad, André; Koch, Holger Martin; Kolossa-Gehring, Marike; Rucic, Enrico; Schmied-Tobies, Maria Irene Hilde; Schwedler, Gerda
Metabolites of di-(2-ethylhexyl) terephthalate (DEHTP), a substitute for ortho-based phthalate plasticisers like di-(2-ethylhexyl) phthalate (DEHP), were analysed in 2112 first-morning void urine samples from children and adolescents aged 3-17 years, participating in the population representative German Environmental Survey on Children and Adolescents, GerES V 2014-2017. The major metabolite 5cx-MEPTP was detected in all urine samples with a geometric mean (GM) of 7.39 (my)g/L, with highest levels in the mg/L range. The GM for the other metabolites were 0.55 (my)g/L for 5OH-MEHTP, 0.54 (my)g/L for 5oxo-MEHTP and below the limit of quantification (LOQ) for 2cx-MMHTP. As already observed for other plasticisers and their substitutes, the youngest children (3-5 years) had 2-2.5-fold higher urinary DEHTP metabolite levels compared to 14-17 years old adolescents. High urinary levels of DEHTP metabolites were associated with high DEHTP concentrations in house dust. None of the samples analysed exceeded the toxicologically derived German human biomonitoring guidance value (HBM-I-Value) of 1.8 mg/L for 5cx-MEPTP. Comparison with DEHTP levels reported in other HBM studies worldwide confirmed a widespread exposure of children, adolescents and adults, with considerably higher exposures (2.6-7 fold) reported in the United States. In GerES V, exposure data for 12 different phthalates and the phthalate substitute DINCH were generated as well. Together with the data for DEHTP presented in this manuscript, GerES V allows a current and comprehensive overview on the concurrent exposure of German children and adolescents to common plasticisers. Further evaluation of aggregate exposure characteristics shall support efforts to reduce chemical hazard burden from plasticisers in Germany and beyond. © 2020 The Author(s).
The exposure of German children and young adults to chemicals of concern
(2013) Apel, Petra; Conrad, André; Fiddicke, Ulrike; Schröter-Kermani, Christa; Schulz, Christine; Seiwert, Margarete
The IMAGE project - a GC-MS/MS analytical method for analysing glyphosate & AMPA in urine
(2020) Connolly, Alison; Koch, Holger Martin; Koslitz, Stephan; Kolossa-Gehring, Marike; Conrad, André
The IMAGE project - Ireland's bioMonitoring assessment of glyphosate exposures
(2020) Connolly, Alison; Koch, Holger Martin; Koslitz, Stephan; Kolossa-Gehring, Marike; Conrad, André