Auflistung nach Autor:in "Gries, Wolfgang"

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DPHP metabolites in urine samples of the German Environmental Specimen Bank from 1999 to 2012
(2014) Schütze, Andre; Gries, Wolfgang; Kolossa-Gehring, Marike; Apel, Petra; Schröter-Kermani, Christa; Brühning, Thomas; Leng, Gabriele; Koch, Holger Martin
The aim of this study was to investigate a possible time trend and status quo of
dipropylheptylphthalate (DPHP) exposure. DPHP is used as a substitute of other high molecular weightphthalates in high temperature applications (e.g cable wires, roofing membranes, etc.). DPHP was selected inthe cooperation project between the German Federal Ministry for Environment (BMU) and the German ChemicalIndustry Association (VCI) due to its listing as High Production Volume (HPV) chemical in the European Union.The BMU-VCI project establishes new human biomonitoring methods and biomarkers for fifty emergingsubstances. 300 urine samples (24-hour voids) from the German Environmental Specimen Bank were analyzedfor three specific, secondary oxidized DPHP metabolites (with hydroxyl, oxo and carboxy modifications of thealkyl side chain). Urine samples were collected in the years 1999, 2003, 2006, 2009 and 2012, 60 samples peryear, from 30 male and 30 female volunteers (age: 21-29 years). The samples were analyzed by liquid/liquidextraction followed by GC-HRMS, which enabled us to distinguish between DPHP and Di-iso-decyl phthalate(DiDP) metabolites. The limit of quantification was between 0.15 ìg/l and 0.3 ìg/l, depending on the metabolite.All samples were blinded before analysis. DPHP metabolites were not detected in the years 1999-2006.Thereafter, detection rates increased from 3.3% in 2009 to 21.7% in 2012. As expected, mono-oxo-propylheptylphthalate (oxo-MPHP) was the most abundant metabolite, with concentrations between Quelle: 24th Annual Meeting ofThe International Society of Exposure Science: Exposure Science Integration to Protect Ecological Systems,Human Well-Being, and Occupational Health; Abstrct Book ISES 2014 / International Society of Exposure Science, Cincinnati: 2014, S.128
Internal exposure of young German adults to di(2-propylheptyl) phthalate (DPHP): Trends in 24-h urine samples from the German Environmental Specimen Bank 1999-2017
(2019) Schmidtkunz, Christoph; Gries, Wolfgang; Kolossa-Gehring, Marike; Weber, Till
Di(2-propylheptyl) phthalate (DPHP) is used as a substitute for high molecular weight phthalates like di(2-ethylhexyl) phthalate (DEHP) which were subjected to authorization under REACh in 2015. An earlier study on the time trend of exposure in human 24-h urine samples from the German Environmental Specimen Bank has revealed that metabolites of DPHP emerged in 2009 and 2012 (Schütze et al., 2015). In order to better assess a potential trend and the present state of exposure to DPHP, we now measured 180 urine samples from the German Environmental Specimen Bank, 60 per year, collected in 2011, 2014 and 2017, randomized and blinded before analysis. Together with the previously analyzed samples, data for a total of 480 samples covering 19 years from 1999 to 2017 was thus generated. We were able to show that DPHP exposure of the studied population, university students from Münster (Northwestern Germany), has remained essentially constant since 2011, after a rapid increase starting around 2009. Even so, urinary metabolite concentrations were always in the low ppb or sub-ppb range, indicating that DPHP exposure of the general population is substantially lower than for other modern plasticizers, and far below levels currently regarded as critical. DPHP is a plasticizer which is mostly used in non-sensitive applications with little probability of close contact to humans. Still, we observed how temporal trends of DPHP exposure largely follow trends of DPHP consumption in the Western European market. Our results hence demonstrate the potential of biomonitoring to sensitively detect the effects of industrial product strategy on the environment, even when biomarkers are present only at trace level. © 2019 Elsevier GmbH. All rights reserved.
New biomarkers for DPHP, 2-MBT and MDI
(2015) Gries, Wolfgang; Leng, Gabriele; Kolossa-Gehring, Marike; Fiddicke, Ulrike
1. Analytical determination of specific 4,4- Methylenediphenyl-diisocyanate (MDI) hemoglobin adducts: MDI is one of the most important isocyanates in the industrial production of polyurethane and other MDI based synthetics. Because of its reactivity it builds adducts with proteins. Analysis of MDI is routinely done by determination of 4,4-methylene dianiline as marker for MDI exposure in urine and blood. This procedure does not allow distinguishing between the source of exposure; MDA or MDI. This method was developed to detect the MDI specific hemoglobin adduct 5-isopropyl-3[4-(4-aminobenzyl)phenyl] hydantoin in human blood.
2. Analytical method for the selective determination of major Di-(2-propylheptyl)-phthalate (DPHP) metabolites in human urine: DPHP is a specific phthalic acid ester of isomeric C10 alcohols and is marketed as plasticizer for polyvinyl chloride . The object of this method development is the sensitive and selective determination of the three major DPHP metabolites mono-2-(propyl-6-hydroxy-heptyl)-phthalate, mono-2-(propyl-6-oxoheptyl)-phthalate and mono-2-(propyl-6-carboxy-hexyl)-phthalate in human urine among those originating from DIDP or DINP. Values up to 3.8 ìg/L OH-MPHP were detected in 51 urines of children during the "Pilot Phase of the 5th German Environmental Survey-Part 1Ł.
3. Rapid and sensitive LC-MS/MS determination of 2-Mercaptobenzothiazole (MBT) in human urine: MBT is one of the most important vulcanization accelerators in the industrial production of rubber. Incorporated MBT is excreted in urine mainly as conjugates. A method was developed to determine the internal MBT dose in human urine. Total MBT is measured after enzymatic hydrolysis followed by application of high pressure liquid chromatography tandem mass spectrometry in positive electrospray ionization mode. In the "Pilot Phase of the 5th German Environmental Survey-Part 1Ł MBT vThe International Societyof Exposure Science: 25th Annual Meeting: Exposures in an Evolving Environment; October 18 - 22, 2015 - Henderson, Nevada, S. alues up to 5.95 ìg/L were analyzed in 51 urines of children.
In: The International Societyof Exposure Science: 25th Annual Meeting: Exposures in an Evolving Environment; October 18 - 22, 2015 - Henderson, Nevada, S.137
New specific and sensitive biomonitoring methods for chemicals of emerging health relevance
(2017) Leng, Gabriele; Fiddicke, Ulrike; Gries, Wolfgang; Kolossa-Gehring, Marike
In this publication the challenges to cope for the aim to obtain innovative biomonitoring methods in our laboratory are visualized for di(2-propylheptyl)phthalate, 2-mercaptobenzothiazole, 3,5-di-tert-butyl-4-hydroxytoluene, 4-nonylphenol, 4-tert-octylphenol, 3-(4-methylbenzylidene)camphor, 4,4'-methylene diphenyl diisocyanate, and Hexabromocyclododecane. For these substances new specific markers were explored based on animal or human kinetic data with urine being the preferred matrix compared to blood. The determination of these markers was complex in all cases, because the sample preparation as well as the detection by high performance liquid chromatography, capillary gas chromatography coupled to tandem mass spectrometers or high resolution mass spectrometry should enable the lowest possible detection limit by use of minimal biological sample volumes. To get a first hint of a possible background level, the analytical methods were applied to urine samples of about 40 persons for each chemical. For Di(2-propylheptyl)phthalate and 2-Mercaptobenzothiazole first results are presented from population biomonitoring. Quelle: http://www.sciencedirect.com